This list includes films from Focus Features, Working Title Films, Universal Film Manufacturing Company, Universal International Pictures, and Universal TV.
| Rank | Title | Year | Director(s) | Overall Rank |
| 101 | The 40 Year Old Virgin | 2005 | Judd Apatow | 1590 |
| 102 | Parenthood | 1989 | Ron Howard | 1591 |
| 103 | Friday Night Lights | 2004 | Peter Berg and Josh Pate | 1612 |
| 104 | Primary Colors | 1998 | Mike Nichols | 1651 |
| 105 | 1941 | 1979 | Steven Spielberg | 1663 |
| 106 | Seabiscuit | 2003 | Gary Ross | 1684 |
| 107 | Land and Freedom2 | 1995 | Ken Loach | 1695 |
| 108 | Conan the Barbarian | 1982 | John Milius | 1715 |
| 109 | The Hurricane | 1999 | Norman Jewison | 1745 |
| 110 | Fear and Loathing in Las Vegas | 1998 | Terry Gilliam | 1753 |
| 111 | The Bingo Long Traveling All-Stars & Motor Kings | 1976 | John Badham | 1760 |
| 112 | Frenzy | 1972 | Alfred Hitchcock | 1763 |
| 113 | The Naked City1 | 1948 | Jules Dassin | 1785 |
| 114 | Flash Gordon | 1936 | Frederick Stephani | 1800 |
| 115 | Mallrats | 1995 | Kevin Smith | 1811 |
| 116 | My Little Chickadee | 1940 | Edward F. Cline | 1829 |
| 117 | All of Me | 1984 | Carl Reiner | 1839 |
| 118 | Imitation of Life | 1934 | John M. Stahl | 1848 |
| 119 | Son of Frankenstein | 1939 | Rowland V. Lee | 1861 |
| 120 | Scent of a Woman | 1992 | Martin Brest | 1871 |
| 121 | Carlito's Way | 1993 | Brian De Palma | 1880 |
| 122 | Monty Python's The Meaning of Life | 1983 | Terry Gilliam and Terry Jones | 1887 |
| 123 | Schizopolis | 1996 | Steven Soderbergh | 1900 |
| 124 | About a Boy2 | 2002 | Chris and Paul Weitz | 1922 |
| 125 | Cinderella Man | 2005 | Ron Howard | 1928 |
| 126 | Reality Bites | 1994 | Ben Stiller | 1981 |
| 127 | American Psycho | 2000 | Mary Harron | 1986 |
| 128 | Fletch | 1985 | Michael Ritchie | 1988 |
| 129 | Serenity | 2005 | Joss Whedon | 1992 |
| 130 | In the Name of the Father | 1993 | Jim Sheridan | 2021 |
| 131 | Happy Gilmore | 1996 | Dennis Dugan | 2061 |
| 132 | Airport | 1970 | George Seaton | 2102 |
| 133 | Munich | 2005 | Steven Spielberg | 2113 |
| 134 | Pride & Prejudice2 3 | 2005 | Joe Wright | 2125 |
| 135 | Jesus Christ Superstar | 1973 | Norman Jewison | 2146 |
| 136 | Sweet Charity | 1969 | Bob Fosse | 2147 |
| 137 | The Man Who Wasn't There2 | 2001 | Joel Coen | 2158 |
| 138 | The Tall Guy2 | 1989 | Mel Smith | 2162 |
| 139 | Criss Cross1 | 1949 | Robert Siodmak | 2171 |
| 140 | Slaughterhouse-Five | 1972 | George Roy Hill | 2188 |
| 141 | Dracula's Daughter | 1936 | Lambert Hillyer | 2191 |
| 142 | Edward II2 | 1991 | Derek Jarman | 2233 |
| 143 | Ulzana's Raid | 1972 | Robert Aldrich | 2261 |
| 144 | Grace of My Heart | 1996 | Allison Anders | 2271 |
| 145 | Gorillas in the Mist: The Story of Dian Fossey | 1988 | Michael Apted | 2273 |
| 146 | Bob Roberts2 | 1992 | Tim Robbins | 2278 |
| 147 | The Nutty Professor | 1996 | Tom Shadyac | 2281 |
| 148 | Clockers | 1995 | Spike Lee | 2282 |
| 149 | Hannibal | 2001 | Ridley Scott | 2291 |
| 150 | King of the Hill | 1993 | Steven Soderbergh | 2294 |
| 151 | Mask | 1985 | Peter Bogdanovich | 2361 |
| 152 | Hellzapoppin' | 1941 | H.C. Potter | 2363 |
| 153 | One True Thing | 1998 | Carl Franklin | 2390 |
| 154 | Charley Varrick | 1973 | Don Siegel | 2398 |
| 155 | Somewhere in Time | 1980 | Jeannot Szwarc | 2401 |
| 156 | Smokey and the Bandit | 1977 | Hal Needham | 2405 |
| 157 | Play Misty for Me | 1971 | Clint Eastwood | 2433 |
| 158 | Dead Men Don't Wear Plaid | 1982 | Carl Reiner | 2460 |
| 159 | Hot Fuzz2 | 2007 | Edgar Wright | 2462 |
| 160 | Counsellor at Law | 1933 | William Wyler | 2470 |
| 161 | Thirteen2 | 2003 | Catherine Hardwicke | 2480 |
| 162 | Son of Dracula | 1943 | Robert Siodmak | 2484 |
| 163 | The Ghost of Frankenstein | 1942 | Erle C. Kenton | 2485 |
| 164 | Bend of the River1 | 1952 | Anthony Mann | 2510 |
1 Produced by Universal International Pictures.
2 Produced by Working Title Films.
3 Produced by Focus Features.
4 Produced by Universal Film Manufacturing Company.
5 Produced by Universal TV.
